Large-scale proteomic analysis of the grapevine leaf apoplastic fluid reveals mainly stress-related proteins and cell wall modifying enzymes.

ABSTRACT:

Background

The extracellular space or apoplast forms a path through the whole plant and acts as an interface with the environment. The apoplast is composed of plant cell wall and space within which apoplastic fluid provides a means of delivering molecules and facilitates intercellular communications. However, the apoplastic fluid extraction from in planta systems remains challenging and this is particularly true for grapevine (Vitis vinifera L.), a worldwide-cultivated fruit plant. Large-scale proteomic analysis reveals the protein content of the grapevine leaf apoplastic fluid and the free interactive proteome map considerably facilitates the study of the grapevine proteome.

Results

To obtain a snapshot of the grapevine apoplastic fluid proteome, a vacuum-infiltration-centr

SUBMITTER: Delaunois B 

PROVIDER: S-EPMC3640900 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature