Cholinergic muscarinic receptor activation augments murine intestinal epithelial cell proliferation and tumorigenesis.

ABSTRACT:

Background

Previously, we showed that M3 muscarinic receptor (M3R; gene name Chrm3) deficiency attenuates murine intestinal neoplasia, supporting the hypothesis that muscarinic receptors play an important role in intestinal tumorigenesis.

Methods

To test this hypothesis, in the present study we treated mice with bethanechol, a non-selective muscarinic receptor agonist without nicotinic receptor activity, and examined its effects on azoxymethane (AOM)-induced colon neoplasia. Mice were provided with drinking water containing 400 μg/mL bethanechol chloride or water without additions (control) for a total of 20 weeks, a period that included the initial 6 weeks when mice received intraperitoneal injections of AOM.

Results

When euthanized at week 20, control mice had 8.0 ±

SUBMITTER: Peng Z 

PROVIDER: S-EPMC3640951 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature