Rapid and automated multidimensional fluorescence microscopy profiling of 3D human breast cultures.

ABSTRACT: Three-dimensional (3D) tissue culture provides a physiologically relevant microenvironment for distinguishing malignant from non-malignant breast cell phenotypes. 3D culture assays can also be used to test novel cancer therapies and predict a differential response to radiation between normal and malignant cells in vivo. However, biological measurements in such complex models are difficult to quantify and current approaches do not allow for in-depth multifaceted assessment of individual colonies or unique sub-populations within the entire culture. This is in part due to the limitations of imaging at a range of depths in 3D culture resulting from optical aberrations and intensity attenuation. Here, we address these limitations by combining sample smearing techniques with high-throughput 2D i

SUBMITTER: Park CC 

PROVIDER: S-EPMC3641787 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature