A compendium of nucleosome and transcript profiles reveals determinants of chromatin architecture and transcription.

ABSTRACT: Nucleosomes in all eukaryotes examined to date adopt a characteristic architecture within genes and play fundamental roles in regulating transcription, yet the identity and precise roles of many of the trans-acting factors responsible for the establishment and maintenance of this organization remain to be identified. We profiled a compendium of 50 yeast strains carrying conditional alleles or complete deletions of genes involved in transcriptional regulation, histone biology, and chromatin remodeling, as well as compounds that target transcription and histone deacetylases, to assess their respective roles in nucleosome positioning and transcription. We find that nucleosome patterning in genes is affected by many factors, including the CAF-1 complex, Spt10, and Spt21, in addition to previou

SUBMITTER: van Bakel H 

PROVIDER: S-EPMC3642058 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature