Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.

ABSTRACT: Released by many eukaryotic cells, the exosomes are 40-100 nm vesicles shown to operate over the complex processes of cell-cell communication. Among the metazoan cell lineages known to generate exosomes is the mononuclear phagocyte lineage, a lineage that parasites such as Leishmania are known to subvert as host cells. We previously reported that mouse macrophage signaling and functions are modified once co-incubated with exoproteome of Leishmania promastigotes. Using mass spectrometry analysis, we were curious to further compare the content of purified exosomes released by the J774 mouse macrophage cell line exposed or not to either LPS or to stationary phase Leishmania mexicana promastigotes. Collectively, our analyses resulted in detection of 248 proteins, ∼50-80% of which were shared a

SUBMITTER: Hassani K 

PROVIDER: S-EPMC3642089 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature