Coherent anti-stokes Raman scattering (CARS) microscopy: a novel technique for imaging the retina.

ABSTRACT:

Purpose

To image the cellular and noncellular structures of the retina in an intact mouse eye without the application of exogenous fluorescent labels using noninvasive, nondestructive techniques.

Methods

Freshly enucleated mouse eyes were imaged using two nonlinear optical techniques: coherent anti-Stokes Raman scattering (CARS) and two-photon autofluorescence (TPAF). Cross sectional transverse sections and sequential flat (en face) sagittal sections were collected from a region of sclera approximately midway between the limbus and optic nerve. Imaging proceeded from the surface of the sclera to a depth of ∼60 μm.

Results

The fluorescent signal from collagen fibers within the sclera was evident in the TPAF channel; the scleral collagen fibers showed no organization an

SUBMITTER: Masihzadeh O 

PROVIDER: S-EPMC3643396 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature