Effects of post-transcriptional regulation on phenotypic noise in Escherichia coli.

ABSTRACT: Cell-to-cell variations in protein abundance, called noise, give rise to phenotypic variability between isogenic cells. Studies of noise have focused on stochasticity introduced at transcription, yet the effects of post-transcriptional regulatory processes on noise remain unknown. We study the effects of RyhB, a small-RNA of Escherichia coli produced on iron stress, on the phenotypic variability of two of its downregulated target proteins, using dual chromosomal fusions to fluorescent reporters and measurements in live individual cells. The total noise of each of the target proteins is remarkably constant over a wide range of RyhB production rates despite cells being in stress. In fact, coordinate downregulation of the two target proteins by RyhB reduces the correlation between their level

SUBMITTER: Arbel-Goren R 

PROVIDER: S-EPMC3643596 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature