High-throughput identification of long-range regulatory elements and their target promoters in the human genome.

ABSTRACT: Enhancer elements are essential for tissue-specific gene regulation during mammalian development. Although these regulatory elements are often distant from their target genes, they affect gene expression by recruiting transcription factors to specific promoter regions. Because of this long-range action, the annotation of enhancer element-target promoter pairs remains elusive. Here, we developed a novel analysis methodology that takes advantage of Hi-C data to comprehensively identify these interactions throughout the human genome. To do this, we used a geometric distribution-based model to identify DNA-DNA interaction hotspots that contact gene promoters with high confidence. We observed that these promoter-interacting hotspots significantly overlap with known enhancer-associated histone m

SUBMITTER: Hwang YC 

PROVIDER: S-EPMC3643598 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature