Robo1 regulates the migration and laminar distribution of upper-layer pyramidal neurons of the cerebral cortex.

ABSTRACT: Laminar organization is a key feature of the mammalian cerebral cortex, but the mechanisms by which final positioning and "inside-out" distribution of neurons are determined remain largely unknown. Here, we demonstrate that Robo1, a member of the family of Roundabout receptors, regulates the correct positioning of layers II/III pyramidal neurons in the neocortex. Specifically, we used RNA interference in mice to suppress the expression of Robo1 in a subset of layers II/III neurons, and observed the positions of these cells at distinct developmental stages. In contrast to control neurons that migrated toward the pial surface by P1, Robo1-suppressed neurons exhibited a delay in entering the cortical plate at respective stages. Unexpectedly, after the first postnatal week, these neurons were

SUBMITTER: Gonda Y 

PROVIDER: S-EPMC3643720 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature