Sequential binary gene ratio tests define a novel molecular diagnostic strategy for malignant pleural mesothelioma.

ABSTRACT:

Purpose

To develop a standardized approach for molecular diagnostics, we used the gene expression ratio bioinformatic technique to design a molecular signature to diagnose malignant pleural mesothelioma (MPM) from among other potentially confounding diagnoses and differentiate the epithelioid from the sarcomatoid histologic subtype of MPM. In addition, we searched for pathways relevant in MPM in comparison with other related cancers to identify unique molecular features in MPM.

Experimental design

We conducted microarray analysis on 113 specimens including MPMs and a spectrum of tumors and benign tissues comprising the differential diagnosis of MPM. We generated a sequential combination of binary gene expression ratio tests able to discriminate MPM from other thoracic malign

SUBMITTER: De Rienzo A 

PROVIDER: S-EPMC3644001 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature