Identification of the Hevea brasiliensis AP2/ERF superfamily by RNA sequencing.

ABSTRACT:

Background

Rubber tree (Hevea brasiliensis) laticifers are the source of natural rubber. Rubber production depends on endogenous and exogenous ethylene (ethephon). AP2/ERF transcription factors, and especially Ethylene-Response Factors, play a crucial role in plant development and response to biotic and abiotic stresses. This study set out to sequence transcript expressed in various tissues using next-generation sequencing and to identify AP2/ERF superfamily in the rubber tree.

Results

The 454 sequencing technique was used to produce five tissue-type transcript libraries (leaf, bark, latex, embryogenic tissues and root). Reads from all libraries were pooled and reassembled to improve mRNA lengths and produce a global library. One hundred and seventy-three AP2/ERF contigs wer

SUBMITTER: Duan C 

PROVIDER: S-EPMC3644242 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature