A microfluidic platform for high-sensitivity, real-time drug screening on C. elegans and parasitic nematodes.

ABSTRACT: This paper describes a new microfluidic platform for screening drugs and their dose response on the locomotion behavior of free living nematodes and parasitic nematodes. The system offers a higher sensitivity drug screening chip which employs a combination of existing and newly developed methods. Real-time observation of the entire drug application process (i.e. the innate pre-exposure locomotion, the transient response during drug exposure and the time-resolved, post-exposure behavior) at a single worm resolution is made possible. The chip enables the monitoring of four nematode parameters (number of worms responsive, number of worms leaving the drug well, average worm velocity and time until unresponsiveness). Each parameter generates an inherently different dose response; allowing for a

SUBMITTER: Carr JA 

PROVIDER: S-EPMC3645292 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature