Protein-DNA complexes are the primary sources of replication fork pausing in Escherichia coli.

ABSTRACT: Replication fork pausing drives genome instability, because any loss of paused replisome activity creates a requirement for reloading of the replication machinery, a potentially mutagenic process. Despite this importance, the relative contributions to fork pausing of different replicative barriers remain unknown. We show here that Deinococcus radiodurans RecD2 helicase inactivates Escherichia coli replisomes that are paused but still functional in vitro, preventing continued fork movement upon barrier removal or bypass, but does not inactivate elongating forks. Using RecD2 to probe replisome pausing in vivo, we demonstrate that most pausing events do not lead to replisome inactivation, that transcription complexes are the primary sources of this pausing, and that an accessory replicative h

SUBMITTER: Gupta MK 

PROVIDER: S-EPMC3645559 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature