Detergent-mediated incorporation of transmembrane proteins in giant unilamellar vesicles with controlled physiological contents.

ABSTRACT: Giant unilamellar vesicles (GUVs) are convenient biomimetic systems of the same size as cells that are increasingly used to quantitatively address biophysical and biochemical processes related to cell functions. However, current approaches to incorporate transmembrane proteins in the membrane of GUVs are limited by the amphiphilic nature or proteins. Here, we report a method to incorporate transmembrane proteins in GUVs, based on concepts developed for detergent-mediated reconstitution in large unilamellar vesicles. Reconstitution is performed either by direct incorporation from proteins purified in detergent micelles or by fusion of purified native vesicles or proteoliposomes in preformed GUVs. Lipid compositions of the membrane and the ionic, protein, or DNA compositions in the internal

SUBMITTER: Dezi M 

PROVIDER: S-EPMC3645586 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature