Dynamics of septin ring and collar formation in Saccharomyces cerevisiae.

ABSTRACT: Although the septin ring and collar in budding yeast were described over 20 years ago, there is still controversy regarding the organization of septin filaments within these structures and about the way in which the ring first forms and about how it converts into a collar at the mother-bud neck. Here we present quantitative analyses of the recruitment of fluorescently-tagged septins to the ring and collar through the cell cycle. Septin ring assembly began several minutes after polarity establishment and this interval was longer in daughter than in mother cells, suggesting asymmetric inheritance of septin regulators. Septins formed an initial faint and irregular ring, which became more regular as septins were recruited at a constant rate. This steady rate of septin recruitment continued for

SUBMITTER: Chen H 

PROVIDER: S-EPMC3645914 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature