Maskiton: Interactive, web-based classification of single-particle electron microscopy images.

ABSTRACT: Electron microscopy (EM) is an important tool for determining the composition, arrangement and structure of biological macromolecules. When studying structurally heterogeneous samples using EM, classification is a critical step toward achieving higher resolution and identifying biologically significant conformations. We have developed an interactive, web-based tool, called Maskiton, for creating custom masks and performing 2D classifications on aligned single-particle EM images. The Maskiton interface makes it considerably easier and faster to explore the significance of heterogeneity in single-particle datasets. Maskiton features include: resumable uploads to facilitate transfer of large datasets to the server, custom mask creation in the browser, continual progress updates, and interacti

SUBMITTER: Yoshioka C 

PROVIDER: S-EPMC3645979 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature