Physiological difference in autophagic flux in macrophages from 2 mouse strains regulates cholesterol ester metabolism.

ABSTRACT:

Objective

DBA/2 apoE(-/-) mice have ≈10-fold larger lesions than AKR apoE(-/-) mice. The objective of this study was to determine whether macrophages from these 2 strains had altered cholesterol metabolism that might play a role in their divergent atherosclerosis susceptibility.

Approach and results

AKR and DBA/2 macrophages incubated with acetylated low-density lipoprotein resulted in higher cholesterol ester (CE) and lower free cholesterol accumulation in the DBA/2 cells. However, these strains had equivalent acetylated low-density lipoprotein uptake and cholesterol esterification activity. Cholesterol efflux from unloaded cells to apolipoprotein A-I or high-density lipoprotein was similar in the 2 strains. However, on acetylated low-density lipoprotein loading, cholestero

SUBMITTER: Robinet P 

PROVIDER: S-EPMC3646371 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature