MiR-29c is downregulated in gastric carcinomas and regulates cell proliferation by targeting RCC2.

ABSTRACT:

Background

Previously, using miRNA microarray, we have found that miR-29c is significantly downregulated in advanced gastric carcinoma. In the present study, we investigated whether miR-29c functions as a tumor-suppressor miRNA in gastric carcinoma cells. For this purpose, we verified the downregulation of miR-29c in gastric carcinoma tissues, and assessed the biological effect of miR-29c on gastric carcinoma cells.

Results

In miR-29c-transfected cells, both proliferation and colony formation ability on soft agar were significantly decreased. Although apoptosis was not induced, BrdU incorporation and the proportion of cells positive for phospho-histone H3 (S10) were significantly decreased in miR-29c-transfected cells, indicating that miR-29c may be involved in the regulatio

SUBMITTER: Matsuo M 

PROVIDER: S-EPMC3646694 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature