Mass spectrometry-guided optimization and characterization of a biologically active transferrin-lysozyme model drug conjugate.

ABSTRACT: Transferrin is a promising drug carrier that has the potential to deliver metals, small organic molecules and therapeutic proteins to cancer cells and/or across physiological barriers (such as the blood-brain barrier). Despite this promise, very few transferrin-based therapeutics have been developed and reached clinical trials. This modest success record can be explained by the complexity and heterogeneity of protein conjugation products, which also pose great challenges to their analytical characterization. In this work, we use lysozyme conjugated to transferrin as a model therapeutic that targets the central nervous system (where its bacteriostatic properties may be exploited to control infection) and develop analytical protocols based on electrospray ionization mass spectrometry to char

SUBMITTER: Nguyen SN 

PROVIDER: S-EPMC3647025 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature