Protein co-migration database (PCoM -DB) for Arabidopsis thylakoids and Synechocystis cells.

ABSTRACT: Protein-protein interactions are critical for most cellular processes; however, many remain to be identified. Here, to comprehensively identify protein complexes in photosynthetic organisms, we applied the recently developed approach of blue native PAGE (BN-PAGE) coupled with LC-MS/MS to the thylakoid proteins of Arabidopsis thaliana and the whole cell proteins of whole cell proteins of Synechocystis sp. PCC 6803. We identified 245 proteins from the purified Arabidopsis thylakoid membranes and 1,458 proteins from the whole cells of Synechocystis using the method. Next, we generated protein migration profiles that were assessed by plotting the label-free estimations of protein abundances versus migration distance in BN-PAGE. Comparisons between the migration profiles of the major photosynth

SUBMITTER: Takabayashi A 

PROVIDER: S-EPMC3647082 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature