A next-generation sequencing method for genotyping-by-sequencing of highly heterozygous autotetraploid potato.

ABSTRACT: Assessment of genomic DNA sequence variation and genotype calling in autotetraploids implies the ability to distinguish among five possible alternative allele copy number states. This study demonstrates the accuracy of genotyping-by-sequencing (GBS) of a large collection of autotetraploid potato cultivars using next-generation sequencing. It is still costly to reach sufficient read depths on a genome wide scale, across the cultivated gene pool. Therefore, we enriched cultivar-specific DNA sequencing libraries using an in-solution hybridisation method (SureSelect). This complexity reduction allowed to confine our study to 807 target genes distributed across the genomes of 83 tetraploid cultivars and one reference (DM 1-3 511). Indexed sequencing libraries were paired-end sequenced in 7 pool

SUBMITTER: Uitdewilligen JG 

PROVIDER: S-EPMC3648547 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature