Protein interactions, post-translational modifications and topologies in human cells.

ABSTRACT: The unique and remarkable physicochemical properties of protein surface topologies give rise to highly specific biomolecular interactions, which form the framework through which living systems are able to carry out their vast array of functions. Technological limitations undermine efforts to probe protein structures and interactions within unperturbed living systems on a large scale. Rapid chemical stabilization of proteins and protein complexes through chemical cross-linking offers the alluring possibility to study details of the protein structure to function relationships as they exist within living cells. Here we apply the latest technological advances in chemical cross-linking combined with mass spectrometry to study protein topologies and interactions from living human cells identifyi

SUBMITTER: Chavez JD 

PROVIDER: S-EPMC3650351 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature