Identification of a mutant α1 Na/K-ATPase that pumps but is defective in signal transduction.

ABSTRACT:

Background

It has not been possible to study the pumping and signaling functions of Na/K-ATPase independently in live cells.

Results

Both cell-free and cell-based assays indicate that the A420P mutation abolishes the Src regulatory function of Na/K-ATPase.

Conclusion

A420P mutant has normal pumping but not signaling function.

Significance

Identification of Src regulation-null mutants is crucial for addressing physiological role of Na/K-ATPase. The α1 Na/K-ATPase possesses both pumping and signaling functions. However, it has not been possible to study these functions independently in live cells. We have identified a 20-amino acid peptide (Ser-415 to Gln-434) (NaKtide) from the nucleotide binding domain of α1 Na/K-ATPase that binds and inhibits Src in vitro. The

SUBMITTER: Lai F 

PROVIDER: S-EPMC3650368 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature