Structure- and modeling-based identification of the adenovirus E4orf4 binding site in the protein phosphatase 2A B55α subunit.

ABSTRACT:

Background

The adenovirus E4orf4 protein must bind protein phosphatase 2A (PP2A) for its functions.

Results

The E4orf4 binding site in PP2A was mapped to the α1,α2 helices of the B55α subunit.

Conclusion

The E4orf4 binding site in PP2A-B55α lies above the substrate binding site and does not overlap it.

Significance

A novel functional significance was assigned to the α1,α2 helices of the PP2A-B55α subunit. The adenovirus E4orf4 protein regulates the progression of viral infection and when expressed outside the context of the virus it induces nonclassical, cancer cell-specific apoptosis. All E4orf4 functions known to date require an interaction between E4orf4 and protein phosphatase 2A (PP2A), which is mediated through PP2A regulatory B subunits. Specifically, an

SUBMITTER: Horowitz B 

PROVIDER: S-EPMC3650409 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature