Degradation and synthesis of β-glucans by a Magnaporthe oryzae endotransglucosylase, a member of the glycoside hydrolase 7 family.

ABSTRACT:

Background

Plant pathogens secrete enzymes that degrade plant cell walls to enhance infection and nutrient acquisition.

Results

A novel endotransglucosylase catalyzes cleavage and transfer of β-glucans and decreases the physical strength of plant cell walls.

Conclusion

Endotransglucosylation causes depolymerization and polymerization of β-glucans, depending on substrate molecular size.

Significance

Enzymatic degradation of plant cell walls is required for wall loosening, which enhances pathogen invasion. A Magnaporthe oryzae enzyme, which was encoded by the Mocel7B gene, was predicted to act on 1,3-1,4-β-glucan degradation and transglycosylation reaction of cellotriose after partial purification from a culture filtrate of M. oryzae cells, followed by liquid chr

SUBMITTER: Takahashi M 

PROVIDER: S-EPMC3650418 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature