Vibrio cholerae VttR(A) and VttR(B) regulatory influences extend beyond the type 3 secretion system genomic island.

ABSTRACT: A subset of non-O1/non-O139 serogroup strains of Vibrio cholerae cause disease using type 3 secretion system (T3SS)-mediated mechanisms. An ∼50-kb genomic island carries genes encoding the T3SS structural apparatus, effector proteins, and two transmembrane transcriptional regulators, VttR(A) and VttR(B), which are ToxR homologues. Previous experiments demonstrated that VttR(A) and VttR(B) are necessary for colonization in vivo and promote bile-dependent T3SS gene expression in vitro. To better understand the scope of genes that are potential targets of VttR(A) and VttR(B) regulation, we performed deep RNA sequencing using O39 serogroup strain AM-19226 and derivatives carrying deletions in vttR(A) and vttR(B) grown in bile. Comparison of the transcript profiles from ΔvttR(A) and ΔvttR(B) mu

SUBMITTER: Chaand M 

PROVIDER: S-EPMC3650523 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature