Multiplexed surrogate analysis of glycotransferase activity in whole biospecimens.

ABSTRACT: Dysregulated glycotransferase enzymes in cancer cells produce aberrant glycans--some of which can help facilitate metastases. Within a cell, individual glycotransferases promiscuously help to construct dozens of unique glycan structures, making it difficult to comprehensively track their activity in biospecimens--especially where they are absent or inactive. Here, we describe an approach to deconstruct glycans in whole biospecimens then analytically pool together resulting monosaccharide-and-linkage-specific degradation products ("glycan nodes") that directly represent the activities of specific glycotransferases. To implement this concept, a reproducible, relative quantitation-based glycan methylation analysis methodology was developed that simultaneously captures information from N-, O-,

SUBMITTER: Borges CR 

PROVIDER: S-EPMC3650733 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature