Separation, immobilization, and biocatalytic utilization of proteins by a supramolecular membrane.

ABSTRACT: Membrane separation of biomolecules and their application in biocatalysis is becoming increasingly important for biotechnology, demanding the development of new biocompatible materials with novel properties. In the present study, an entirely noncovalent water-based material is used as a membrane for size-selective separation, immobilization, and biocatalytic utilization of proteins. The membrane shows stable performance under physiological conditions, allowing filtration of protein mixtures with a 150 kDa molecular weight cutoff (∼8 nm hydrodynamic diameter cutoff). Due to the biocompatibility of the membrane, filtered proteins stay functionally active and retained proteins can be partially recovered. Upon filtration, large enzymes become immobilized within the membrane. They exhibit stabl

SUBMITTER: Krieg E 

PROVIDER: S-EPMC3651134 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature