The use of native chemical functional groups presented by wound beds for the covalent attachment of polymeric microcarriers of bioactive factors.

ABSTRACT: The development of versatile methods that provide spatial and temporal control over the presentation of physical and biochemical cues on wound beds can lead to new therapeutic approaches that expedite wound healing by favorably influencing cellular behaviors. Toward that goal, we report that native chemical functional groups presented by wound beds can be utilized for direct covalent attachment of polymeric microbeads. Specifically, we demonstrated the covalent attachment of maleimide-functionalized and catechol-functionalized microbeads, made of either polystyrene (non-degradable) or poly(lactic-co-glycolic acid) ((PLGA), degradable), to sulfhydryl and amine groups present on porcine dermis used here as an ex vivo model wound bed. A pronounced increase (10-70 fold) in the density and pers

SUBMITTER: Jain R 

PROVIDER: S-EPMC3651840 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature