Evolutionary history of black grouse major histocompatibility complex class IIB genes revealed through single locus sequence-based genotyping.

ABSTRACT:

Background

Gene duplications are frequently observed in the Major Histocompatibility Complex (MHC) of many species, and as a consequence loci belonging to the same MHC class are often too similar to tell apart. In birds, single locus genotyping of MHC genes has proven difficult due to concerted evolution homogenizing sequences at different loci. But studies on evolutionary history, mode of selection and heterozygosity correlations on the MHC cannot be performed before it is possible to analyse duplicated genes separately. In this study we investigate the architecture and evolution of the MHC class IIB genes in black grouse. We developed a sequence-based genotyping method for separate amplification of the two black grouse MHC class IIB genes BLB1 and BLB2. Based on this approach we

SUBMITTER: Strand T 

PROVIDER: S-EPMC3652749 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature