Radiosensitivity in breast cancer assessed by the histone γ-H2AX and 53BP1 foci.

ABSTRACT:

Background

High expression of constitutive histone γ-H2AX, a sensitive marker of DNA damage, might be indicative of defective DNA repair pathway or genomic instability. 53BP1 (p53-binding protein 1) is a conserved checkpoint protein with properties of a DNA double-strand breaks sensor. This study explores the relationship between the clinical radiosensitivity of tumor patients and the expression/induction of γ-H2AX and 53BP1 in vitro.

Methods

Using immunostaining, we assessed spontaneous and radiation-induced foci of γ-H2AX and 53 BP1 in peripheral blood mononuclear cells derived from unselected breast cancer (BC) patients (n=57) undergoing radiotherapy (RT). Cells from apparently healthy donors (n=12) served as references.

Results

Non-irradiated cells from controls a

SUBMITTER: Djuzenova CS 

PROVIDER: S-EPMC3653697 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature