Genome-wide promoter methylation profile of human testis and epididymis: identified from cell-free seminal DNA.

ABSTRACT:

Background

DNA methylation analysis is useful for investigation of male fertility in mammals, whereas the reliance on tissues limits the research on human. We have previously found the presence of high concentration of cell-free seminal DNA (cfsDNA) in human semen. We proposed that some testis and epididymis-specific methylated promoters could be detected in human cfsDNA, and thus hold promise as noninvasive epigenetic biomarkers for male infertility, of which most cases are caused by defects in testicular sperm production or epididymal sperm maturation.

Results

The ejaculate of successfully vasectomized men does not contain any secretion from testis and epididymis. Here we compared genome-wide promoter methylation profiles in cfsDNA between health donors and post-vasectomy

SUBMITTER: Wu C 

PROVIDER: S-EPMC3653781 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature