A deep analysis of the small non-coding RNA population in Schistosoma japonicum eggs.

ABSTRACT:

Background

Schistosoma japonicum is a parasitic flatworm that causes zoonotic schistosomiasis. The typical outcome of schistosomiasis is hepatic granuloma and fibrosis, which is primarily induced by soluble egg-derived antigens. Although schistosomal eggs represent an important pathogenic stage to the host, the biology of this critical stage is largely unknown. We previously investigated the expression profiles of sncRNAs during different developmental stages of this parasite. However, using small RNA extracted from egg-deposited liver tissues generated limited information about sncRNAs in eggs. Here, we characterized the complete small RNAome in this stage of the parasite after optimization of RNA purification.

Methodology and principal findings

A library, SjE, was construc

SUBMITTER: Cai P 

PROVIDER: S-EPMC3653858 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature