Phenotypical analysis of atypical PKCs in vivo function display a compensatory system at mouse embryonic day 7.5.

ABSTRACT:

Background

The atypical protein kinases C (PKC) isoforms ι/λ and ζ play crucial roles in many cellular processes including development, cell proliferation, differentiation and cell survival. Possible redundancy between the two isoforms has always been an issue since most biochemical tools do not differentiate between the two proteins. Thus, much effort has been made during the last decades to characterize the functions of aPKCs using gene targeting approaches and depletion studies. However, little is known about the specific roles of each isoform in mouse development.

Methodology/principal findings

To evaluate the importance of PKCι in mouse development we designed PKCι deletion mutants using the gene targeting approach. We show that the deletion of PKCι, results in a reduce

SUBMITTER: Seidl S 

PROVIDER: S-EPMC3653893 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature