Benzo[a]pyrene decreases global and gene specific DNA methylation during zebrafish development.

ABSTRACT: DNA methylation is important for gene regulation and is vulnerable to early-life exposure to environmental contaminants. We found that direct waterborne benzo[a]pyrene (BaP) exposure at 24μg/L from 2.5 to 96hpf to zebrafish embryos significantly decreased global cytosine methylation by 44.8% and promoter methylation in vasa by 17%. Consequently, vasa expression was significantly increased by 33%. In contrast, BaP exposure at environmentally relevant concentrations did not change CpG island methylation or gene expression in cancer genes such as ras-association domain family member 1 (rassf1), telomerase reverse transcriptase (tert), c-jun, and c-myca. Similarly, BaP did not change gene expression of DNA methyltransferase 1 (dnmt1) and glycine N-methyltransferase (gnmt). While total DNMT act

SUBMITTER: Fang X 

PROVIDER: S-EPMC3654064 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature