Antagonism of the histamine H4 receptor reduces LPS-induced TNF production in vivo.

ABSTRACT:

Objective

Antagonism of the histamine H4 receptor (H4R) has been shown to be anti-inflammatory in a number of preclinical disease models, however the exact mechanisms behind this are still being uncovered. In vitro, the receptor interacts with TLR and impacts inflammatory mediator production from a number of different cell types. Here it is shown that this interaction also occurs in vivo.

Materials and methods

Wild-type and H4R deficient BALB/c mice received an i.p. injection of LPS in PBS in conjunction with p.o. JNJ 7777120 or JNJ 28307474 (H4R antagonists). Two hours later blood was collected and TNF was measured.

Results

Two different H4R antagonists inhibited LPS-induced TNF production in mice and this production was also reduced in H4R-deficient mice. The TNF mR

SUBMITTER: Cowden JM 

PROVIDER: S-EPMC3654183 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature