Hepatic gene expression profiling in Nrf2 knockout mice after long-term high-fat diet-induced obesity.

ABSTRACT:

Introduction

The transcription factor NFE2-related factor 2 (Nrf2) is a central regulator of antioxidant and detoxification gene expression in response to electrophilic or oxidative stress. Nrf2 has recently been shown to cross-talk with metabolic pathways, and its gene deletion protected mice from high-fat-diet-(HFD-) induced obesity and insulin resistance. This study aimed to identify potential Nrf2-regulated genes of metabolic interest by comparing gene expression profiles of livers of wild-type (WT) versus Nrf2 knockout (Nrf2-KO) mice after a long-term HFD.

Methods

WT and Nrf2-KO mice were fed an HFD for 180 days; total RNA was prepared from liver and used for microarray analysis and quantitative real-time RT-PCR (qRT-PCR).

Results

The microarray analysis identifi

SUBMITTER: Chartoumpekis DV 

PROVIDER: S-EPMC3654373 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature