Utilizing spectral counting to quantitatively characterize tandem removal of abundant proteins (TRAP) in human plasma.

ABSTRACT: Biomarker discovery efforts in serum and plasma are greatly hindered by the presence of high abundance proteins that prevent the detection and quantification of less abundant, yet biologically significant, proteins. The most common method for addressing this problem is to specifically remove the few abundant proteins through immunoaffinity depletion/subtraction. Herein, we improved upon this method by utilizing multiple depletion columns in series, so as to increase the efficiency of the abundant protein removal and augment the detection/identification of less abundant plasma proteins. Spectral counting was utilized to make quantitative comparisons between undepleted plasma, plasma depleted with a single depletion column, and plasma depleted using two or three depletion columns in tandem.

SUBMITTER: Shuford CM 

PROVIDER: S-EPMC3654688 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature