In vivo protein complex topologies: sights through a cross-linking lens.

ABSTRACT: Proteins are a remarkable class of molecules that exhibit wide diversity of shapes or topological features that underpin protein interactions and give rise to biological function. In addition to quantitation of abundance levels of proteins in biological systems under a variety of conditions, the field of proteome research has as a primary mission the assignment of function for proteins and if possible, illumination of factors that enable function. For many years, chemical cross-linking methods have been used to provide structural data on single purified proteins and purified protein complexes. However, these methods also offer the alluring possibility to extend capabilities to complex biological samples such as cell lysates or intact living cells where proteins may exhibit native topologic

SUBMITTER: Bruce JE 

PROVIDER: S-EPMC3654877 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature