Metabolic engineering of Escherichia coli for high-specificity production of isoprenol and prenol as next generation of biofuels.

ABSTRACT:

Background

The isopentenols, including isoprenol and prenol, are excellent alternative fuels. However, they are not compounds largely accumulated in natural organism. The need for the next generation of biofuels with better physical and chemical properties impels us to develop biosynthetic routes for the production of isoprenol and prenol from renewable sugar. In this study, we use the heterogenous mevalonate-dependent (MVA) isoprenoid pathway for the synthesis of isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) intermediates, and then convert IPP and DMAPP to isoprenol and prenol, respectively.

Results

A mevalonate titer of 1.7 g/L was obtained by constructing an efficient MVA upper pathway in engineered E. coli. Different phosphatases and pyrophospha

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC3654967 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature