Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production.

ABSTRACT:

Background

The ability of fungal cellobiose dehydrogenase (CDH) to generate H2O2 in-situ is highly interesting for biotechnological applications like cotton bleaching, laundry detergents or antimicrobial functionalization of medical devices. CDH's ability to directly use polysaccharide derived mono- and oligosaccharides as substrates is a considerable advantage compared to other oxidases such as glucose oxidase which are limited to monosaccharides. However CDH's low activity with oxygen as electron acceptor hampers its industrial use for H2O2 production. A CDH variant with increased oxygen reactivity is therefore of high importance for biotechnological application. Uniform expression levels and an easy to use screening assay is a necessity to facilitate screening for CDH variants w

SUBMITTER: Sygmund C 

PROVIDER: S-EPMC3654988 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature