Short-term genome stability of serial Clostridium difficile ribotype 027 isolates in an experimental gut model and recurrent human disease.

ABSTRACT:

Background

Clostridium difficile whole genome sequencing has the potential to identify related isolates, even among otherwise indistinguishable strains, but interpretation depends on understanding genomic variation within isolates and individuals.

Methods

Serial isolates from two scenarios were whole genome sequenced. Firstly, 62 isolates from 29 timepoints from three in vitro gut models, inoculated with a NAP1/027 strain. Secondly, 122 isolates from 44 patients (2-8 samples/patient) with mostly recurrent/on-going symptomatic NAP-1/027 C. difficile infection. Reference-based mapping was used to identify single nucleotide variants (SNVs).

Results

Across three gut model inductions, two with antibiotic treatment, total 137 days, only two new SNVs became established. Pre-

SUBMITTER: Eyre DW 

PROVIDER: S-EPMC3655144 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature