Fitness costs of synonymous mutations in the rpsT gene can be compensated by restoring mRNA base pairing.

ABSTRACT: We previously reported that the distribution of fitness effects for non-synonymous and synonymous mutations in Salmonella typhimurium ribosomal proteins S20 and L1 are similar, suggesting that fitness constraints are present at the level of mRNA. Here we explore the hypothesis that synonymous mutations confer their fitness-reducing effect by alterating the secondary structure of the mRNA. To this end, we constructed a set of synonymous substitutions in the rpsT gene, encoding ribosomal protein S20, that are located in predicted paired regions in the mRNA and measured their effect on bacterial fitness. Our results show that for 3/9 cases tested, the reduced fitness conferred by a synonymous mutation could be fully or partly restored by introducing a second synonymous substitution that resto

SUBMITTER: Lind PA 

PROVIDER: S-EPMC3655191 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature