Quantitative Proteomics via High Resolution MS Quantification: Capabilities and Limitations.

ABSTRACT: Recent improvements in the mass accuracy and resolution of mass spectrometers have led to renewed interest in label-free quantification using data from the primary mass spectrum (MS1) acquired from data-dependent proteomics experiments. The capacity for higher specificity quantification of peptides from samples enriched for proteins of biological interest offers distinct advantages for hypothesis generating experiments relative to immunoassay detection methods or prespecified peptide ions measured by multiple reaction monitoring (MRM) approaches. Here we describe an evaluation of different methods to post-process peptide level quantification information to support protein level inference. We characterize the methods by examining their ability to recover a known dilution of a standard prote

SUBMITTER: Higgs RE 

PROVIDER: S-EPMC3655581 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature