Claudin-5 controls intercellular barriers of human dermal microvascular but not human umbilical vein endothelial cells.

ABSTRACT:

Objective

To assess the role claudin-5, an endothelial cell (EC) tight junction protein, plays in establishing basal permeability levels in humans by comparing claudin-5 expression levels in situ and analyzing junctional organization and function in 2 widely used models of cultured ECs, namely human dermal microvascular (HDM)ECs and human umbilical vein (HUV)ECs.

Methods and results

By immunofluorescence microscopy, ECs more highly express claudin-5 (but equivalently express vascular endothelial-cadherin) in human dermal capillaries versus postcapillary venules and in umbilical and coronary arteries versus veins, correlating with known segmental differences in tight junction frequencies and permeability barriers. Postconfluent cultured HDMECs express more claudin-5 (but equi

SUBMITTER: Kluger MS 

PROVIDER: S-EPMC3655806 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature