High-resolution transcriptome maps reveal strain-specific regulatory features of multiple Campylobacter jejuni isolates.

ABSTRACT: Campylobacter jejuni is currently the leading cause of bacterial gastroenteritis in humans. Comparison of multiple Campylobacter strains revealed a high genetic and phenotypic diversity. However, little is known about differences in transcriptome organization, gene expression, and small RNA (sRNA) repertoires. Here we present the first comparative primary transcriptome analysis based on the differential RNA-seq (dRNA-seq) of four C. jejuni isolates. Our approach includes a novel, generic method for the automated annotation of transcriptional start sites (TSS), which allowed us to provide genome-wide promoter maps in the analyzed strains. These global TSS maps are refined through the integration of a SuperGenome approach that allows for a comparative TSS annotation by mapping RNA-seq data o

SUBMITTER: Dugar G 

PROVIDER: S-EPMC3656092 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature