DNA topoisomerase II is involved in regulation of cyst wall protein genes and differentiation in Giardia lamblia.

ABSTRACT: The protozoan Giardia lamblia differentiates into infectious cysts within the human intestinal tract for disease transmission. Expression of the cyst wall protein (cwp) genes increases with similar kinetics during encystation. However, little is known how their gene regulation shares common mechanisms. DNA topoisomerases maintain normal topology of genomic DNA. They are necessary for cell proliferation and tissue development as they are involved in transcription, DNA replication, and chromosome condensation. A putative topoisomerase II (topo II) gene has been identified in the G. lamblia genome. We asked whether Topo II could regulate Giardia encystation. We found that Topo II was present in cell nuclei and its gene was up-regulated during encystation. Topo II has typical ATPase and DNA cl

SUBMITTER: Lin BC 

PROVIDER: S-EPMC3656124 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature