Rapid, simple and sensitive detection of Q fever by loop-mediated isothermal amplification of the htpAB gene.

ABSTRACT:

Background

Q fever is the most widespread zoonosis, and domestic animals are the most common sources of transmission. It is not only difficult to distinguish from other febrile diseases because of the lack of specific clinical manifestations in humans, but it is also difficult to identify the disease in C. burnetii-carrying animals because of the lack of identifiable features. Conventional serodiagnosis requires sera from the acute and convalescent stages of infection, which are unavailable at early diagnosis. Nested PCR and real-time PCR require equipment. In this study, we developed a Loop-Mediated Isothermal Amplification (LAMP) assay to identify C. burnetii rapidly and sensitively.

Methods

A universal LAMP primer set was designed to detect the repeated sequence IS1111a o

SUBMITTER: Pan L 

PROVIDER: S-EPMC3656153 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature