The outer surface lipoprotein VolA mediates utilization of exogenous lipids by Vibrio cholerae.

ABSTRACT:

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Previous work from our laboratory showed that the Gram-negative aquatic pathogen Vibrio cholerae can take up a much wider repertoire of fatty acids than other Gram-negative organisms. The current work elaborated on the ability of V. cholerae to exploit an even more diverse pool of lipid nutrients from its environment. We have demonstrated that the bacterium can use lysophosphatidylcholine as a metabolite for growth. Using a combination of thin-layer chromatography and mass spectrometry, we also showed that lysophosphatidylcholine-derived fatty acid moieties can be used for remodeling the V. cholerae membrane architecture. Furthermore, we have identified a lysophospholipase, VolA (Vibrio outer membrane lysophospholipase A), required for these activities. The enzyme is wel

SUBMITTER: Pride AC 

PROVIDER: S-EPMC3656444 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature