Reproductive status alters transcriptomic response to infection in female Drosophila melanogaster.

ABSTRACT: Mating and consequent reproduction significantly reduce the ability of female Drosophila melanogaster to defend against systemic bacterial infection. The goal of the present study was to identify genes likely to inform the mechanism of this post-mating immunosuppression. We used microarrays to contrast genome-wide transcript levels in virgin vs. mated females before and after infection. Because the immunosuppressive effect of mating is contingent on the presence of a germline in females, we repeated the entire experiment by using female mutants that do not form a germline. We found that multiple genes involved in egg production show reduced expression in response to infection, and that this reduction is stronger in virgins than it is in mated females. In germline-less females, expression o

SUBMITTER: Short SM 

PROVIDER: S-EPMC3656730 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature